Monospecific antibody and method of production using as antigen an isoform of the human fad synthetase

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Abstract

The antiserum is directed against human FAD synthase or FMN:ATP adenylyl transferase, a collection of enzymatic isoforms involved in FAD biosynthesis. It specifically reacts with the isoforms in human (and rat) biopsies, cellular extracts, plasma, and blood lysates, liquor. It is able to immune-react with both domains contained in the longest isoforms. FAD synthase deficiency and/or impairment have been linked to certain human neuro-muscular diseases, such as the RR-MADD, which, if early diagnosed, can be treated with the simple ingestion of riboflavin in high doses. Early diagnosis requires measurements of the human enzyme level. Main advantages Methods for measuring FAD synthase activity require specific competences in enzymology and spectrofluorometric assay, thus are time and money consuming. The antibody permits a rapid and simple analysis feasible by any laboratories. Innovative aspects The patented antibody is the best molecular test available. It is of interest for research and diagnostic purposes. It is expected that several research group and companies should purchase the antiserum. Differently from other antisera raised against the same enzyme, our antiserum recognizes each FAD synthase isoform. It has been tested in dot blot, immuno-blotting and immuno-fluorescence analysis. The antiserum also recognizes isoforms from model organisms like S. cerevisiae and C. elegans

Classe Tecnologica

C - Chemistry and Metallurgy

Patent Office

World Intellectual Property Organization

Numero Deposito

PCT/IT2009/000062

Anno Deposito

2009

Anno Concessione

Inventori Pugliesi

Tutti gli inventori

  • Barile Maria
  • Torchietti Enza Maria
  • Indiveri Cesare
  • Galluccio Michele

Titolari pugliesi

Tutti i titolari

  • Università Degli Studi Di Bari Aldo Moro
  • Università Della Calabria