Abstract

The spoilage potential of Brettanomyces bruxellensis in wine is strongly connected with the aptitude ofthis yeast to enter in a Viable But Non Culturable (VBNC) state when exposed to the harsh wineconditions. In this work, we characterized the VBNC behaviour of seven strains of B. bruxellensisrepresenting a regional intraspecific biodiversity, reporting conclusive evidence for the assessment ofVBNC as a strain-dependent character. The VBNC behaviour was monitored by fluorescein diacetatestaining/flow cytometry for eleven days after addition of 0.4, 0.6, 0.8, 1 and 1.2 mg/L of molecular SO2(entrance in the VBNC state) and after SO2 removal (exit from the VBNC state). Furthermore, onerepresentative strain was selected and RNA-seq analysis performed after exposure to 1.2 mg/L SO2 andduring the recovery phase. 30 and 1634 genes were identified as differentially expressed followingVBNC entrance and 'resuscitation', respectively. The results reported strongly suggested that theentrance in the SO2-induced VBNC state in B. bruxellensis is associated with both, sulfite toxicity andoxidative stress response, confirming the crucial role of genes/proteins involved in redox cell homeostasis. Among the genes induced during recovery, the expression of genes involved in carbohydrate metabolism and encoding heat shock proteins, as well as enriched categories including amino acid transport and transporter activity was observed. The evidences of a general repression of genes involved in DNA replication suggest the occurrence of a true resuscitation of cell rather than a simple regrowth.

Autore Pugliese

• Grieco Francesco

Tutti gli autori

• V. Capozzi ; M.R. Di Toro ; F. Grieco ; V. Michelotti ; M. Salma; A. Lamontanara ; P. Russo ; L.Orrù ; H. Alexandre; G. Spano

Titolo volume/Rivista

Food microbiology

Anno di pubblicazione

2016

ISSN

0740-0020

ISBN

Non Disponibile

Numero di citazioni Wos

Nessuna citazione

Ultimo Aggiornamento Citazioni

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Numero di citazioni Scopus

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Ultimo Aggiornamento Citazioni

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Settori ERC

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Codici ASJC

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