Abstract

The photosynthetic reaction center (RC) from the Rhodobacter sphaeroides bacterium has been covalently bioconjugated with a NIR-emitting fluorophore (AE800) whose synthesis was specifically tailored to act as artificial antenna harvesting light in the entire visible region. AE800 has a broad absorption spectrum with peaks centered in the absorption gaps of the RC and its emission overlaps the most intense RC absorption bands, ensuring a consistent increase of the protein optical cross section. The covalent hybrid AE800-RC is stable and fully functional. The energy collected by the artificial antenna is transferred to the protein via FRET mechanism, and the hybrid system outperforms by a noteworthy 30% the overall photochemical activity of the native protein under the entire range of visible light. This improvement in the optical characteristic of the photoenzyme demonstrates the effectiveness of the bioconjugation approach as a suitable route to new biohybrid materials for energy conversion, photocatalysis, and biosensing. © 2016 American Chemical Society.

Autore Pugliese

• Milano Francesco , Hassan Omar Omar , Trotta Massimo

Tutti gli autori

• Hassan Omar O.; La Gatta S.; Tangorra R.R.; Milano F.; Ragni R.; Operamolla A.; Argazzi R.; Chiorboli C.; Agostiano A.; Trotta M.; Farinola G.M.

Titolo volume/Rivista

Bioconjugate chemistry

Anno di pubblicazione

2016

ISSN

1520-4812

ISBN

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Numero di citazioni Wos

Nessuna citazione

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Numero di citazioni Scopus

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Settori ERC

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Codici ASJC

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