Polymorphic positions of Grapevine yellow speckle viroid-2 (GYSVd-2) in Iranian sequence variants
Abstract
To date, GYSVd-2 has been found only in few countries and grapevine is the only known natural host of GYSVd-2, which has restricted distribution in world viticulture. In this study, three GYSVd-2 isolates from three distinct regions of northwest Iran were molecularly characterized. The Iranian GYSVd-2 variants were composed of 360-363 nucleotides sharing a high sequence identity of 96-100% with each other, 97-99% with the reference sequence (NC-003612) and 99-100% with other GYSVd-2 sequences. In total, six new variants were identified with most nucleotide changes with respect to the reference variant located in the terminal left (TL) domain of the proposed rod-like secondary structure. Three and five variants showed the deletion of A356 and U363 in the TL domain, respectively. Also, substitutions of G359 by U, U360 by C and C361 by U in five variants were found.In contrast, limited nucleotide changes were observed in other domains. In silico analysis showed that some of the nucleotide substitutions would affect the predicted secondary structures but possible biological roles on viroid replication and pathogenesis are unknown and would require further investigations.
Autore Pugliese
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Hajizadeh M.; Sokhandan-Bashir N. ; Navarro B.; Di Serio F.
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Anno di pubblicazione
2013
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Settori ERC
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Codici ASJC
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