Abstract

Purpose: The aim of the study was to evaluate the vascular anti-inflammatory effects of polyphenolic extracts from two typical South Italy red wines, the specific contribution of individual polyphenols and the underlying mechanisms of action.Methods: Human endothelial cells were incubated with increasing concentrations (1-50 ?g/mL) of Primitivo and Negroamaro polyphenolic extracts (PWPE and NWPE, respectively) or pure polyphenols (1-25 ?mol/L), including hydroxycinnamic acids (p-coumaric, caffeic and caftaric acids), flavonols (kaempferol, quercetin, myricetin) or stilbenes (trans-resveratrol, trans-piceid) before stimulation withlipopolysaccharide. Through multiple assays, we analyzed the endothelial-monocyte adhesion, theendothelial expression of adhesion molecules (ICAM-1, VCAM-1 and E-Selectin), monocytechemoattractant protein-1 (MCP-1) and macrophage colony-stimulating factor (M-CSF), as well as ROSintracellular levels and the activation of NF-?B and AP-1.Results: Both PWPE and NWPE, already at 1 ?g/mL, inhibited monocyte adhesion to stimulated endothelial cells, a key event in triggering vascular inflammation. They down-regulated the expression of adhesion molecules, ICAM-1, VCAM-1, E-Selectin, as well as MCP-1 and M-CSF, at mRNA and protein levels. All polyphenols reduced intracellular ROS, and everything, except caftaric acid, inhibited the endothelial expression of adhesion molecules and MCP-1, although with different potency. Flavonols and resveratrol significantly reduced also the endothelial expression and release of M-CSF. The decrease in endothelial inflammatory gene expression was related to the inhibition of NF-?B and AP-1 activation but not to intracellular oxidative stress.Conclusions: This study showed multiple anti-inflammatory and anti-atherosclerotic properties of red wine polyphenolic extracts and indentified specific bioactive polyphenols which could counteract inflammatory diseases including atherosclerosis.

Autore Pugliese

• Giovinazzo Giovanna , Scoditti Egeria , Carluccio Maria Annunziata

Tutti gli autori

• N. Calabriso ; E. Scoditti ; M. Massaro ; M. Pellegrino ; C. Storelli ; I. Ingrosso ; G. Giovinazzo ; M.A. Carluccio

Titolo volume/Rivista

European journal of nutrition

Anno di pubblicazione

2016

ISSN

1436-6207

ISBN

Non Disponibile

Numero di citazioni Wos

Nessuna citazione

Ultimo Aggiornamento Citazioni

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Numero di citazioni Scopus

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Ultimo Aggiornamento Citazioni

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Settori ERC

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Codici ASJC

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