Abstract

Novel photosensitizing film based on the natural hybrid polymer Chitosan/2-hydroxy-propyl-b-Cyclodextrin(CH/CD) is synthesized introducing Chlorophyll a (CH/CD/Chla) as a photoactive agent forpossible application in antimicrobial photodynamic therapy (PDT). The polymer absorbs visible light, inturn able to generate reactive oxygen species (ROS) and, therefore it can be used as environmentalfriendly and biodegradable polymeric photosensitizer (PS). The modified film is characterized by meansof different spectroscopic, calorimetric, diffraction techniques and microscopic imaging methodsincluding time-resolved absorption spectroscopy. UVeVis, FTIR-ATR and X-ray Photoelectron Spectroscopy(XPS) analyses suggest that Chla shows a strong affinity toward Chitosan introducing interactionswith amino groups present on the polymer chains. Nanosecond laser flash photolysis technique providesevidence for the population of the excited triplet state of Chla. Photogeneration of singlet oxygen isdemonstrated by both direct detection by using infrared luminescence spectroscopy and chemicalmethods based on the use of suitable traps. Scanning Electron Microscopy (SEM), Atomic Force Microscopy(AFM) and Differential Scanning Calorimetry (DSC) analyses confirm also the occurrence ofstructural changes both on the film surface and within the film layer induced by the insertion of thepigment. Moreover, X-ray Diffraction data (XRD) shows the existence of an amorphous phase for thechitosan films in all the compared conditions.

Autore Pugliese

• Giannini Cinzia , Sibillano Teresa

Tutti gli autori

• V. Rizzi; P. Fini; F. Fanelli; T. Placido; P. Semeraro; T. Sibillano; A. Fraix; Salvatore Sortino; A. Agostiano; C. Giannini; P. Cosma

Titolo volume/Rivista

Food hydrocolloids

Anno di pubblicazione

2016

ISSN

0268-005X

ISBN

Non Disponibile

Numero di citazioni Wos

Nessuna citazione

Ultimo Aggiornamento Citazioni

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Numero di citazioni Scopus

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Ultimo Aggiornamento Citazioni

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Settori ERC

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Codici ASJC

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