Isolation and identification of entomopathogenic fungi and their evaluation against Tribolium confusum, Sitophilus zeamais, and Rhyzopertha dominica in stored maize

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Abstract

The objective of this study was to isolate andidentify native entomopathogenic fungi from differentcomponents of maize agroecosystem, and evaluate theirvirulence against Tribolium confusum, Rhyzopertha dominica,and Sitophilus zeamais, three insect pest vectors ofaflatoxigenic fungi. Paecilomyces and Metarhizium werethe most abundant genera isolated from the soil. Identificationof fungal cultures by DNA extraction, amplification,and sequencing showed that all isolates macro- andmicromorphological identified as P. lilacinus were Purpureocilliumlilacinum. The isolate JQ926223 showed thelowest LT50 for T. confusum (4, 66 days) and R. dominica(9, 38 days), and the isolate JQ926212 demonstrated similarLT50 for the three insects evaluated between the rangeof 11, 7 to 14, 95 days. Maximum mortality rate wasobserved for the isolate JQ926223. The isolates of Purpureocilliumlilacinum JQ926223 and JQ926212 may beconsidered good candidates for biologic control in theecosystem of stored maize.

Autore Pugliese

Tutti gli autori

  • P. Barra; L. Rosso; A. Nesci; M. Etcheverry

Titolo volume/Rivista

Journal of pest science

Anno di pubblicazione

2013

ISSN

1612-4758

ISBN

Non Disponibile

Numero di citazioni Wos

Nessuna citazione

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Numero di citazioni Scopus

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Settori ERC

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Codici ASJC

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