Abstract

Sanitary selection and certification of olive cultivars require sensitive diagnostic methods and effectivesanitation protocols. Although much attention has been paid in the past few years to the developmentof diagnostic tools for reliable virus identification, the need to define a common andstandardized diagnostic protocol led to the implementation of a ring test among nine Italian diagnosticlaboratories. A one-step RT-PCR protocol and different primer sets, targeting the most commonolive viruses covered by phytosanitary rules, were tested in each laboratory, using the same batch ofpositive and healthy controls as well as the same amplification conditions and reaction components.The one-step RT-PCR, performed using several specific primer sets, was able efficiently to detectthe target viruses in all laboratories. Furthermore, a one-step RT-PCR protocol was used successfullyfor the first time for detection of Tobacco necrosis virus (TNV) and Olive mild mosaic virus(OMMV). Results showed that all target viruses were not uniformly distributed in the canopy, andthat at least two subsets of samples must be collected from each plant. This standardized protocol isnow being used to produce nuclear stocks for 70 different Italian olive cultivars, in the framework ofthe national project OLVIVA, which involves 25 national research institutions.

Autore Pugliese

• Saponari Maria

Tutti gli autori

• G. Loconsole; M. Saponari; F. Faggioli; G. Albanese; H. Bouyahia; T. Elbeaino;A. Materazzi; M. Nuzzaci; V. Prota; G. Romanazzi; N. Trisciuzzi; V. Savino

Titolo volume/Rivista

Bulletin OEPP

Anno di pubblicazione

2010

ISSN

0250-8052

ISBN

Non Disponibile

Numero di citazioni Wos

Nessuna citazione

Ultimo Aggiornamento Citazioni

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Numero di citazioni Scopus

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Ultimo Aggiornamento Citazioni

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Settori ERC

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Codici ASJC

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