Engineering resistance against viroids

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Abstract

Viroids, the smallest infectious agents endowed with autonomous replication, are tiny single-stranded circular RNAs (~250 to 400 nt) without protein-coding ability that, despite their simplicity, infect and often cause disease in herbaceous and woody plants of economic relevance. To mitigate the resulting losses, several strategies have been developed, the most effective of which include: firstly, search for naturally resistant cultivars and breeding for resistance, secondly, induced resistance by pre-infection with mild strains, thirdly, ribonucleases targeting double-stranded RNAs and catalytic antibodies endowed with intrinsic ribonuclease activity, fourthly, antisense, and sense, RNAs, fifthly, catalytic antisense RNAs derived from hammerhead ribozymes, and sixthly, hairpin RNAs and artificial small RNAs for RNA interference. The mechanisms underpinning these strategies, most of which have been implemented via genetic transformation, together with their present results and future potential, are the subject of this review.

Autore Pugliese

Tutti gli autori

  • Flores R.; Navarro B.; Kovalskaya N.; Hammond R.W.; Di Serio F.

Titolo volume/Rivista

Current opinion in virology

Anno di pubblicazione

2017

ISSN

1879-6257

ISBN

Non Disponibile

Numero di citazioni Wos

Nessuna citazione

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Numero di citazioni Scopus

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Settori ERC

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Codici ASJC

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