Abstract

A method for the determination of fumonisin B1 (FB1) and B2 (FB2) in different commercial maize-based products for infants and young children was developed and tested in a limited validation study involving 3 laboratories. The method used extraction at 55 °C with an acidic mixture of methanol-acetonitrile-phosphate/citrate buffer, clean-up through immunoaffinity column and fumonisin determination by high performance liquid chromatography with automated pre-column derivatisation with o-phthaldialdehyde. Recovery experiments were performed at five spiking levels in the ranges of 80-800 ?g/kg FB1 and 20-200 ?g/kg FB2. Mean recoveries ranged from 83 to 97% for FB1 and from 61 to 78% for FB2. Relative standard deviations for within-laboratory repeatability (RSDr) ranged from 5 to 12% for FB1 and from 8 to 13% for FB2, whereas relative standard deviation for between-laboratory reproducibility (RSDR) ranged from 6 to 10% for FB1 and from 9 to 16% for FB2. The limit of quantification of the method (signal to noise ratio of 6) was 2.8 ?g/kg for FB1 and 2.2 ?g/kg for FB2. Fumonisins were found in 6 out of 19 maize-based baby foods obtained from the Italian retail market at levels up to 53 ?g/kg.

Autore Pugliese

• De Girolamo Annalisa , Gambacorta Lucia , Solfrizzo Michele

Tutti gli autori

• De Girolamo A.; Pereboom-de Frauw D.; Sizoo E.; van Egmond H.P.; Gambacorta L.; Bouten K.; Stroka J.; Visconti A.;Solfrizzo M.

Titolo volume/Rivista

World mycotoxin journal

Anno di pubblicazione

2010

ISSN

1875-0796

ISBN

Non Disponibile

Numero di citazioni Wos

Nessuna citazione

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Numero di citazioni Scopus

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Settori ERC

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Codici ASJC

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