Abstract

Morphological and molecular analyses confirmed the presence of Pratylenchus hippeastri in regulatory samples collectedin commercial bromeliad operations from genera Guzmania, Neoregelia and Vriesea in central and south Florida, USA. Specimens ofP. hippeastri from bromeliads contained males which were not detected in the type population from amaryllis and are described hereinfor the first time. The rDNA sequences of these males matched those of P. hippeastri female type material. Pratylenchus hippeastri androot-lesion nematodes from several hosts in Florida were characterised at the morphological and molecular level, whereas other samplesfrom Russia and South Africa were characterised at the molecular level only. Sequence and phylogenetic analysis using the ITS rRNAgene of these root-lesion nematodes revealed the presence of eight putative new species (spH1-H8) closely related to P. hippeastri.Here we describe two Florida representatives of the amphimictic root-lesion nematodes from Bahia grass (N1) and maidencane (N2),previously characterised by Inserra et al. in 1996 and Duncan et al. in 1999, as two new species phylogenetically related to P. hippeastriand named P. floridensis n. sp. and P. parafloridensis n. sp., respectively. The small round or oval (rarely rectangular and occasionallyoblong) and enlarged spermatheca and the bluntly pointed or subacute tail with smooth and occasionally indented terminus separateP. floridensis n. sp. from P. parafloridensis n. sp., which has a quadrangular spermatheca and a subhemispherical or bluntly pointed tailwith generally smooth and rarely indented terminus. However, these characters may overlap in some specimens making a morphologicalseparation problematic without the use of molecular analysis. The close phylogenetic relationships shared by the species characterisedin this study indicate that they are representatives of a P. hippeastri species complex.

Autore Pugliese

• Troccoli Alberto , De Luca Francesca

Tutti gli autori

• De Luca F.; Troccoli A.; Duncan L.W.; Subbotin S.A.; Waeyenberge L.; Moens M.; Inserra R.N.

Titolo volume/Rivista

Nematology

Anno di pubblicazione

2010

ISSN

1388-5545

ISBN

Non Disponibile

Numero di citazioni Wos

Nessuna citazione

Ultimo Aggiornamento Citazioni

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Numero di citazioni Scopus

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Ultimo Aggiornamento Citazioni

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Settori ERC

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Codici ASJC

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