Abstract

Rat liver mitochondria were isolated in parallel in two different isolation buffers: a standard buffer containing mannitol/sucrose and a nearly physiological KCl based solution. The two different organelle preparations were comparatively characterized by respiratory activity, heme content, microsomal and Golgi contamination, electron microscopy and lipid analyses. The substitution of saccharides with KCl in the isolation buffer does not induce the formation of mitoplasts or disruption of mitochondria. Mitochondria isolated in KCl buffer are coupled and able to maintain a stable transmembrane charge separation. A number of biochemical and functional differences between the two organelle preparations are described; in particular KCl mitochondria exhibit lower cardiolipin content and smaller intracristal compartments in comparison with the standard mitochondrial preparation.

Autore Pugliese

• Corcelli Angela , Mastrodonato Maria , Lopalco Patrizia

Tutti gli autori

• CORCELLI A.;MASTRODONATO M.;LOPALCO P.;LOPALCO P.L.

Titolo volume/Rivista

Non Disponibile

Anno di pubblicazione

2010

ISSN

0006-3002

ISBN

Non Disponibile

Numero di citazioni Wos

17

Ultimo Aggiornamento Citazioni

Non Disponibile

Numero di citazioni Scopus

15

Ultimo Aggiornamento Citazioni

Non Disponibile

Settori ERC

Non Disponibile

Codici ASJC

Non Disponibile