Abstract

The application of high-performance Next-Generation Sequencing (NGS) technologies is widely used to characterize case-control comparison studies for RNA transcripts, such as mRNAs and small non-coding RNAs. The first step in the analy- sis strategies is mapping NGS reads against a reference database, and a critical issue is choosing how to deal with multiread problem. In this paper we present a novel ap- proach to represent and quantify read mapping ambiguities through the use of fuzzy sets and possibility theory. The aim of this work is to obtain a list of candidate differential expression events, ordered by significance, providing a description of the uncertainty of the results due to the multiread issue. A preliminary experiment on a case-control study of human endobronchial biopsies resulted in the identification of 9 genes with possible differential expression, four of them with an uncertain fold change. This result was con- firmed by FDR adjusted Fisher’s test, while the same data processed with DESeq2 did not provide significant differences between case and control.

Autore Pugliese

• Mencar Corrado

Tutti gli autori

• MENCAR C.

Titolo volume/Rivista

Non Disponibile

Anno di pubblicazione

2016

ISSN

1611-3349

ISBN

9783319443317

Numero di citazioni Wos

Nessuna citazione

Ultimo Aggiornamento Citazioni

Non Disponibile

Numero di citazioni Scopus

Non Disponibile

Ultimo Aggiornamento Citazioni

Non Disponibile

Settori ERC

Non Disponibile

Codici ASJC

Non Disponibile