Coupled TLC and MALDI-TOF/MS analyses of the lipid extract of the hyperthermophilic archaeon Pyrococcus furiosus.

Abstract

The lipidome of the marine hyperthermophilic archaeon Pyrococcus furiosus was studied by means of combined thin-layer chromatography and MALDI-TOF/MS analyses of the total lipid extract. 80–90% of the major polar lipids were represented by archaeol lipids (diethers) and the remaining part by caldarchaeol lipids (tetraethers). The direct analysis of lipids on chromatography plate showed the presence of the diphytanylglycerol analogues of phosphatidylinositol and phosphatidylglycerol, the N-acetylglucosamine-diphytanylglycerol phosphate plus some caldarchaeol lipids different from those previously described. In addition, evidence for the presence of the dimeric ether lipid cardiolipin is reported, suggesting that cardiolipins are ubiquitous in archaea.


Tutti gli autori

  • CORCELLI A.;LOPALCO P.;LOBASSO S.;LOBASSO S.

Titolo volume/Rivista

Non Disponibile


Anno di pubblicazione

2012

ISSN

1472-3646

ISBN

Non Disponibile


Numero di citazioni Wos

11

Ultimo Aggiornamento Citazioni

Non Disponibile


Numero di citazioni Scopus

13

Ultimo Aggiornamento Citazioni

Non Disponibile


Settori ERC

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Codici ASJC

Non Disponibile