Abstract

The aim of this study was to characterize nanoparticles (NPs) composed of chitosan (CS) and evaluate their potential for brain delivery of the neurotransmitter Dopamine (DA). For this purpose, CS based NPs were incubated with DA at two different concentrations giving rise to nanocarriers denoted as DA/CSNPs (1) and DA/CSNPs (5), respectively. X-ray Photoelectron Spectroscopy (XPS) analysis confirmed that DA was adsorbed onto the external surface of such NPs. The cytotoxic effect of the CSNPs and DA/CSNPs was assessed using the MTT test and it was found that the nanovectors are less cytotoxic than the neurotransmitter DA after 3 h of incubation time. Transport studies across MDCKII-MDR1 cell line showed that DA/CSNPs (5) give rise to a significant transport enhancing effect compared with the control and greater than the corresponding DA/CSNPs (1). Measurement of reactive oxygen species (ROS) suggested a low DA/CSNPs neurotoxicity after 3 h. In vivo brain microdialysis experiments in rat showed that intraperitoneal acute administration of DA/CSNPs (5) (6-12 mg/kg) induced a dose-dependent increase in striatal DA output. Thus, these CS nanoparticles represent an interesting technological platform for DA brain delivery and, hence, may be useful for Parkinson's disease treatment. (C) 2011 Elsevier B.V. All rights reserved.

Autore Pugliese

• Trapani Adriana , De Giglio Elvira , Agrimi Gennaro , Denora Nunzio

Tutti gli autori

• TRAPANI A.;DE GIGLIO E.;AGRIMI G.;TRAPANI G.;DENORA N.

Titolo volume/Rivista

Non Disponibile

Anno di pubblicazione

2011

ISSN

0378-5173

ISBN

Non Disponibile

Numero di citazioni Wos

86

Ultimo Aggiornamento Citazioni

Non Disponibile

Numero di citazioni Scopus

102

Ultimo Aggiornamento Citazioni

Non Disponibile

Settori ERC

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Codici ASJC

Non Disponibile