Abstract

Vacuolar Sorting Determinants (VSDs) have been extensively studied in plants but the mechanisms for the accumulation of storage proteins in somatic tissues are not yet fully understood. In this work we used two mutated versions of well-documented vacuolar fluorescent reporters, a GFP fusion in frame with the C-terminal VSD of tobacco chitinase (GFPChi) and an N-terminal fusion in frame with the sequence-specific VSD of the barley cysteine protease aleurain (AleuGFP). The GFP sequence was mutated to present an N-glycosylation site at the amino-acid position 133. The reporters were transiently expressed in Nicotiana tabacum protoplasts and agroinfiltrated in Nicotiana benthamiana leaves and their distribution was identical to that of the non-glycosylated versions. With the glycosylated GFPs we could highlight a differential ENDO-H sensitivity and therefore differential glycan modifications. This finding suggests two different and independent routes to the vacuole for the two reporters. BFA also had a differential effect on the two markers and further, inhibition of COPII trafficking by a specific dominant-negative mutant (NtSar1h74I) confirmed that GFPChi transport from the ER to the vacuole is not fully dependent on the Golgi apparatus. (C) 2013 Elsevier Masson SAS. All rights reserved.

Autore Pugliese

• Piro Gabriella , Di Sansebastiano Gian Pietro , Montefusco Anna

Tutti gli autori

• Stigliano E. , Faraco M. , Neuhaus J.M. , Montefusco A. , Dalessandro G. , Piro G. , Di Sansebastiano G.P.

Titolo volume/Rivista

PLANT PHYSIOLOGY AND BIOCHEMISTRY

Anno di pubblicazione

2013

ISSN

0981-9428

ISBN

Non Disponibile

Numero di citazioni Wos

15

Ultimo Aggiornamento Citazioni

28/04/2018

Numero di citazioni Scopus

15

Ultimo Aggiornamento Citazioni

28/04/2018

Settori ERC

Non Disponibile

Codici ASJC

Non Disponibile