Abstract

The results of the present study suggest that DmTpc1 is actively implicated in the specific uptake of free cytoplasmic Pt bonded nucleotides, and therefore could be linked to the mechanism of action of some platinum-based antitumor drugs. Although DmTpc1 has a low affinity for model [Pt(dien)(N7-5′-dGTP)] and cis-[Pt(NH3)2(py)(N7-5′-dGTP)] compared to dATP it's well known that DNA platination level of few metal atoms per double-stranded molecule may account for the pharmacological activity of platinum based antitumor drugs. This is the first investigation where it has been demonstrated that a mitochondrial carrier is directly involved in the transport of metalated purines related with the cisplatin mechanism of action. Moreover it is shown as a lower hindrance of nucleotide bonded platinum complexes could strongly enhance mitochondrial uptake. Furthermore, a new application of ICP-AES addressed to measure the transport of metalated nucleobases, by using a recombinant protein reconstituted into liposomes, has been here, for the first time, developed and compared with a standard technique such as the liquid scintillation counting.

Autore Pugliese

• Benedetti Michele , Capobianco Loredana , Fanizzi Francesco Paolo

Tutti gli autori

• C. Carrisi , D. Antonucci , P. Lunetti , D. Migoni , C. R. Girelli , V. Dolce , F. P. Fanizzi , M. Benedetti , L. Capobianco

Titolo volume/Rivista

JOURNAL OF INORGANIC BIOCHEMISTRY

Anno di pubblicazione

2014

ISSN

0162-0134

ISBN

Non Disponibile

Numero di citazioni Wos

4

Ultimo Aggiornamento Citazioni

28/04/2018

Numero di citazioni Scopus

5

Ultimo Aggiornamento Citazioni

28/04/2018

Settori ERC

Non Disponibile

Codici ASJC

Non Disponibile