Abstract

Phospholipid biosynthesis exerts an important role in the proliferation of tumor cells; however, the regulation of the proteins involved in this context still remains to be fully evaluated. SLC37A1 protein belongs to a small family of sugar-phosphate/phosphate exchangers. The sequence homology with the bacterial glycerol-3-phosphate transporter (30%) suggests that SLC37A1 might be able to catalyze an exchange of glycerol-3-phosphate against phosphate. Glycerol-3-phosphate, found in different cellular compartments, is a fundamental substrate in phospholipid biosynthesis. In the present study, we demonstrate for the first time that epidermal growth factor (EGF) transactivates SLC37A1 promoter sequence and induces SLC37A1 mRNA, and protein expression through the EGFR/MAPK/ Fos transduction pathway in ER-negative SkBr3 breast cancer cells. These findings were corroborated by comparable results obtained in ER-positive endometrial Ishikawa tumor cells. Interestingly, we also show that SLC37A1 protein localizes in the endoplasmic reticulum, hence supporting its possible involvement in phospholipid biosynthesis. On the basis of our data, the up-regulation of SLC37A1 gene expression should be included among the well-known stimulatory action exerted by EGF in breast cancer cells. In addition, further studies are required to provide evidence concerning the potential role of EGFmediated SLC37A1 induction in breast tumor cells.

Autore Pugliese

• Capobianco Loredana

Tutti gli autori

• D. Iacopetta , R. Lappano , A.R. Cappello , M. Madeo , E. M. De Francesco , A. Santoro , R. Curcio , L. Capobianco , V. Pezzi , M. Maggiolini , V. Dolce

Titolo volume/Rivista

BREAST CANCER RESEARCH AND TREATMENT

Anno di pubblicazione

2010

ISSN

0167-6806

ISBN

Non Disponibile

Numero di citazioni Wos

17

Ultimo Aggiornamento Citazioni

25/04/2018

Numero di citazioni Scopus

19

Ultimo Aggiornamento Citazioni

24/04/2018

Settori ERC

Non Disponibile

Codici ASJC

Non Disponibile