Abstract

A flow-injection impedimetric immunosensor for the sensitive, direct and label-free detection of cholera toxin is reported. A limit of detection smaller than 10 pM was achieved, a value thousands of times lower than the lethal dose. The developed chips fulfil the requirement of low cost and quick reply of the assay and are expected to enable field screening, prompt diagnosis and medical intervention without the need of specialized personnel and expensive equipment, a perspective of special relevance for use in developing countries. Since the chip layout includes two sensing areas each one with a 2 × 2 sensor array, our biochips can allow statistical or (alternatively) multiplex analysis of biorecognition events between antibodies immobilized on each working electrode and different antigens flowing into the chamber.

Autore Pugliese

• Maruccio Giuseppe , Rinaldi Rosaria

Tutti gli autori

• M. S. Chiriacò , E. Primiceri , E. D'Amone , R. E. Ionescu , R. Rinaldi , G. Maruccio

Titolo volume/Rivista

LAB ON A CHIP

Anno di pubblicazione

2011

ISSN

1473-0197

ISBN

Non Disponibile

Numero di citazioni Wos

35

Ultimo Aggiornamento Citazioni

28/04/2018

Numero di citazioni Scopus

38

Ultimo Aggiornamento Citazioni

28/04/2018

Settori ERC

Non Disponibile

Codici ASJC

Non Disponibile