The main aim of TRANSBIO is the implementation of an innovative cascading concept for the valorisation of sub-products from fruit and vegetable processing industry using environmental friendly biotechnological solutions like fermentation and enzyme-conversion strategies to obtain valuable bioproducts like plastics (PHB), nutraceuticals / platform chemical succinic acid and enzymes for detergent applications. TRANSBIO will characterize and select appropriate by-products from fruit and vegetable processing industry, followed by adapted pre-treatment and enzymatic hydrolysis procedures to obtain fermentable sugars for microbial fermentation. In order to obtain a broad application potential for the by-products selected, the project will investigate three different fermentation strategies – submerged cultivation (SmF) in liquid media (bacteria, yeasts) and solid state fermentation (SSF) (fungi). Beside optimisation and up-scaling of fermentation Protocols, down-stream processing will be developed keeping in mind economical feasibility, sustainability as well as end-product formation (intracellular, extra cellular). The procedures will be optimised for extra cellular succinic acid production in SmF using novel non-conventional yeast strains and extracelluar enzyme formation in SSF under utilisation of fungi as well as intracellular PHB formation in SmF with bacteria. The obtained PHB will be tested for their packaging application, enzymes will be proved for detergent utilisation and succinic acid will be purified for food application. Beside, original and pre-treated by-products as well as remaining biomass from fermentation strategies will be tested for their potential to be used as feedstock for biogas production via anaerobic digestion.