Worldwide diffusion of Fig latent virus 1 in fig accessions and its detection by serological and molecular tools
Abstract
A virus with filamentous articles ca. 700 nm long, denoted Fig latent virus 1 (FLV-1) is widespread in Apulian(southern Italy) fig orchards, in trees showing or not mosaic symptoms and in symptomless seedlings. The virus wastransmitted by sap inoculation to a very restricted range of herbaceous hosts without inducing apparent symptoms andwas transmitted through fig seeds to a very high percentage (80 to 100 %). It was successfully purified from root tissues of infected figs. A virus-specific antiserum raised in rabbits, proved useful for its detection in fig leaf dips byimmunosorbent electron microscopy (ISEM), Western Blot, dot immuno-binding (DIBA), ELISA.The viral genomestructure resembles that of members of the genus Trichovirus in the family Flexiviridae.
Autore Pugliese
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Gattoni G.; Minafra A.; Castellano M.A.; De Stradis A; Boscia D.; Elbeaino T.; Digiaro M.; Martelli G.P.
Titolo volume/Rivista
Julius Khun Archiv
Anno di pubblicazione
2010
ISSN
1868-9892
ISBN
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