Validation of a lateral flow immunoassay for the rapid determination of aflatoxins in maize by solvent free extraction

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Abstract

The analytical performances of a lateral flow immunoassay (AFLA-V AQUA(TM), Vicam a Waters Business) forthe determination of aflatoxins in maize were evaluated according to Commission Regulation (EU) No. 519/2014. This case study showed how a critical evaluation of validation results can provide a quite informativepicture of kit performances for its use for different purposes. The validation study, performed at aflatoxin B1levels encompassing EU maximum permitted levels (up to 8 ug kg-1), provided information on the methodprecision profile, cut off values, and false suspect and false negative rates. The total precision expressed asrelative standard deviation varied from 14 to 29% for contaminated samples. Cut off values calculatedconsidering 2 ug kg-1 or 4 mg kg-1 as the screening target concentration were 1.24 and 2.18 ug kg-1,respectively, whereas the resultant false suspect rate for blanks was 42% and 8%, respectively. The falsenegative rate for samples containing the analyte at higher concentrations was found to be lower than1%. Analysis of naturally contaminated maize samples performed by the lateral flow immunoassay andthe AOAC official method revealed a good correlation (parameters of the linear regression: r = 0.97,slope = 0.96) between the obtained results. A satisfactory agreement between reference values and testresults was also obtained in the analysis of reference materials. Finally, a QC protocol based onguidelines suggested in the EU regulation was designed to verify kit performances over time and in newproduction lots.

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Tutti gli autori

  • V.M.T. Lattanzio; N. Guarducci; S. Powers; B. Ciasca; M. Pascale; C. von Holst

Titolo volume/Rivista

Analytical methods

Anno di pubblicazione

2017

ISSN

1759-9679

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