Abstract

The synthesis of partially hydrolyzed fumonisins (PHFB1 and PHFB2) and hydrolyzed fumonisins (HFB1 and HFB2) by chemicalhydrolysis of pure fumonisins (FB1 and FB2) is reported together with the isolation and characterization by liquidchromatography-high-resolution mass spectrometry (LC-HRMS). Two structural isomers of partially hydrolyzed forms ofFB1 and FB2 were identified, namely PHFB1a and PHFB1b and PHFB2a and PHFB2b. Reaction yields were 21% for PHFB1(sum of the two isomers), 52% for HFB1, 31% for PHFB2 (sum of the two isomers) and 30% for HFB2. Purity of each isolatedcompound was >98%.An LC-HRMS method for the simultaneous determination of fumonisins and their partially and totally hydrolyzedderivatives was applied to 24 naturally contaminated samples of maize and maize-based products. The majority of samples(18 out of 24) were contaminated with fumonisins B1 and B2. Fumonisins co-occurred with both partially hydrolyzed andhydrolyzed fumonisins in four nixtamalized samples (three masa flours and one tortilla chips). Co-occurrence of fumonisins withpartially hydrolyzed fumonisins was also recorded in one sample ofmaize kernels and four samples ofmaize-based products (i.e.maize meal, cous-cous, corn-cakes and cornflakes). Mycotoxins levels ranged from 60 to 5700 ?g/kg for fumonisins (sum of FB1and FB2), from 10 to 210 ?g/kg for partially hydrolyzed fumonisins (sum of PHFB1 and PHFB2) and from 30 to 200 ?g/kg forhydrolyzed fumonisins (sum of HFB1 and HFB2). This is the first report of the isolation of PHFB2 and the co-occurrence of FB1,FB2, PHFB1, PHFB2, HFB1 and HFB2 in maize products. Considering the growing use of nixtamalized and maize-based products,the monitoring of fumonisins and their partially and totally hydrolyzed forms in these products may represent an importantcontributing factor in evaluating the relevant human risk exposure.

Autore Pugliese

• De Girolamo Annalisa , Pascale Michelangelo , Schena Roberto , Lattanzio Veronica Maria Teresa

Tutti gli autori

• De Girolamo A.; Lattanzio V.M.T.; Schena R.; Visconti A.; Pascale M.

Titolo volume/Rivista

Journal of mass spectrometry

Anno di pubblicazione

2014

ISSN

1076-5174

ISBN

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Numero di citazioni Wos

Nessuna citazione

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Settori ERC

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Codici ASJC

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