Abstract

MicroRNAs are post-transcriptional regulators of eukaryotic organisms that function through mRNA degradation or suppression of translation. Artificial microRNA (amiRNA) act in similar way and this technology has recently attracted much attention as a tool against plant virus infections. In this study, a construct assembled on the Vitis vinifera miR166f (vvi-miR166f) pre-miRNA was used to construct an amiRNA cassette by replacing the 21 nucleotides (nt) of the miR166f sequence with a 21 nt sequence from the Grapevine virus A (GVA) genome. Two amiRNAs cassettes were engineered with 21 nt sequences targeting GVA ORF1 and ORF5. Transient expression of these amiRNAs in Nicotiana benthamiana led to various levels of resistance indicating amiRNA technology as a potential tool for GVA control.

Autore Pugliese

• Saldarelli Pasquale

Tutti gli autori

• Roumi V.; Afsharifar A.R.; Saldarelli P.; Niazi A.; Martelli G.P.; Izadpanah K.

Titolo volume/Rivista

Journal of plant pathology

Anno di pubblicazione

2012

ISSN

1125-4653

ISBN

Non Disponibile

Numero di citazioni Wos

Nessuna citazione

Ultimo Aggiornamento Citazioni

Non Disponibile

Numero di citazioni Scopus

Non Disponibile

Ultimo Aggiornamento Citazioni

Non Disponibile

Settori ERC

Non Disponibile

Codici ASJC

Non Disponibile