Abstract

Nitrogen (N) is a key macronutrient representing a limiting factor for plant growth and developmentand affects productivity in wheat. In this study, durum wheat response to N chronic starvation duringgrain filling was investigated through a transcriptomic approach in roots, leaves/stems, flag leaf andspikes of cv. Svevo. Nitrogen stress negatively influenced plant height, tillering, flag leaf area, spike andseed traits, and total N content. RNA-seq data revealed 4,626 differentially expressed genes (DEGs).Most transcriptomic changes were observed in roots, with 3,270 DEGs, while 963 were found in leaves/stems, 470 in flag leaf, and 355 in spike tissues. A total of 799 gene ontology (GO) terms were identified,180 and 619 among the upregulated and downregulated genes, respectively. Among the mostaddressed GO categories, N compound metabolism, carbon metabolism, and photosynthesis weremostly represented. Interesting DEGs, such as N transporters, genes involved in N assimilation, alongwith transcription factors, protein kinases and other genes related to stress were highlighted. Theseresults provide valuable information about the transcriptomic response to chronic N stress in durumwheat, which could be useful for future improvement of N use efficiency

Autore Pugliese

• Sonnante Gabriella

Tutti gli autori

• Curci P.L.; Aiese Cigliano R.; Zuluaga D.L.; Janni M.; Sanseverino W.; Sonnante G.

Titolo volume/Rivista

Scientific reports

Anno di pubblicazione

2017

ISSN

2045-2322

ISBN

Non Disponibile

Numero di citazioni Wos

Nessuna citazione

Ultimo Aggiornamento Citazioni

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Numero di citazioni Scopus

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Settori ERC

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Codici ASJC

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