Transcriptome analysis shows differential gene expression in the saprotrophic to parasitic transition of Pochonia chlamydosporia

Torna indietro

Abstract

Expression profiles were identified in the fungusPochonia chlamydosporia, a biological control agent ofplant parasitic nematodes, through a cDNA-amplifiedfragment length polymorphism approach. Two isolates withdifferent host ranges, IMI 380407 and IMI 331547, wereassayed in conditions of saprotrophic-to-parasitic transition,through in vitro assays. Gene expression profiles from threedifferent nutritional conditions and four sampling timeswere generated, with eggs of host nematodes Globoderapallida and Meloidogyne incognita. Expression of transcriptschanged in RNA fingerprints obtained underdifferent nutritional stresses (starvation in presence/absenceof eggs, or rich growth media). Transcript derived fragments(TDFs) obtained from the expression profilescorresponded to 6,800 products. A subset was sequencedand their expression profile confirmed through RT PCR. Atotal of 57 TDFs were selected for further analysis, basedon similarities to translated or annotated sequences. Genesexpressed during egg parasitism for both IMI 380407 andIMI 331547 were involved in metabolic functions, cellularsignal regulation, cellular transport, regulation of geneexpression, DNA repair, and other unknown functions.Multivariate analysis of TDF expression showed threegroups for IMI 380407 and one for IMI 331547, eachcharacterized by expression of genes related to eggsparasitism. Common amplification profiles among TDFclusters from both isolates also reflected a pool ofconstitutive genes, not affected by the nutritional conditionsand nematode associations, related to general metabolicfunctions. The differential expression of parasitism relatedgenes suggest a network of induced/repressed products,playing a role in fungal signaling and infection, with partialoverlaps in host infection and parasitism traits.

Autore Pugliese

Tutti gli autori

  • Rosso L.C.; Finetti-Sialer M.M.; Hirsch P.R.; Ciancio A.; Kerry B.R.; Clark I.M.

Titolo volume/Rivista

Applied microbiology and biotechnology

Anno di pubblicazione

2011

ISSN

0175-7598

ISBN

Non Disponibile

Numero di citazioni Wos

Nessuna citazione

Ultimo Aggiornamento Citazioni

Non Disponibile

Numero di citazioni Scopus

Non Disponibile

Ultimo Aggiornamento Citazioni

Non Disponibile

Settori ERC

Non Disponibile

Codici ASJC

Non Disponibile