Abstract

Fusarium is a genus frequently contaminating different agricultural products and is also a renown producer of Trichothecenes1. This group of mycotoxins can be further subdivided into subgroups with T-2/HT-2 toxins and deoxynivalenol being the main representatives of the type A and B respectively. In general Trichothecenes are responsible for a wide variety of toxic effects in humans and animals. Within the type class A, in particular, T-2 and HT-2 toxins represent the most acutely toxic compounds2 and have been lately reviewed by EFSA3. In human health risk assessment, ingestion of contaminated food is considered the major route of human exposure to these compounds although only a smaller amount will be further available for absorption during its transit along the gut4. In vitro digestion models have been used to evaluate mycotoxins bioaccessibility during the intestinal transit. As a result, the bioaccessibility can be calculated representing a measure for the assessment of mycotoxin bioavailability in food. Some investigations have been already carried out in the past by our group in order to assess T-2 and HT-2 bioaccessibility in bread chosen as reference food model5 In this note we describe a study aimed to investigate toxicity of gastro-duodenal digests of mycotoxins contaminated bread samples collected along the digestion time-course, assayed on RPMI lymphoid B cell line, as a sensitive cellular model. In parallel, a chemical quantification of T-2 and HT-2 toxins contaminating the bread digests was accomplished along the gastric and duodenal phase. The digestive fluids undergoing chemical and toxicological analysis, were collected at the beginning and end of the gastric phase, and after completion of the duodenal phase Results proved that a correlation between HT-2 content and toxicity did exist in digestive fluids although a more persistent toxic activity was noticed in the later stage of the duodenal phase.

Autore Pugliese

• Minervini Fiorenza , Monaci Linda

Tutti gli autori

• L. Monaci; A. Garbetta; E. De Angelis; A. Visconti; F. Minervini.

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Anno di pubblicazione

2015

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