Toxic mechanisms induced by fumonisin B1 mycotoxin on human intestinal cell line

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Abstract

The gastrointestinal tract is the main target ofexposure to mycotoxin fumonisin B1 (FB1), common naturalcontaminant in food. Previous studies reported thatproliferating cells are more sensitive than confluent cells tothe toxic effect of FB1. This study aims to investigate, bydose- and time-dependent experiments on human colonproliferating intestinal cell line (HT-29), the modificationsinduced by FB1 at concentrations ranging from 0.25 to69 lM. The choice of highest FB1 concentration consideredthe low toxicity previously reported on intestinal celllines, whereas the lowest one corresponded to the lowerFBs levels permitted by European Commission Regulation.Different functional parameters were tested such as cellproliferation, oxidative status, immunomodulatory effectand changes in membrane microviscosity. In addition FB1-FITC localization in this cell line was assessed by usingconfocal laser scanning microscopy. Lipid peroxidationinduction was the main and early (12 h) effect induced byFB1 at concentrations ranging from 0.5 to 69 lM, followedby inhibition of cell proliferation (up to 8.6 lM), theimmunomodulatory effect (up to 17.2 lM), by assessingIL-8 secretion, and increase in membrane microviscosity(up to 34.5 lM). The toxic effects observed in differentfunctional parameters were not dose-dependent and couldbe the consequence of the FB1 intracytoplasmatic localizationas confirmed by confocal microscopy results. Thedifferent timescales and concentrations active of differentfunctional parameters could suggest different cellular targetsof FB1.

Autore Pugliese

Tutti gli autori

  • Minervini F.; Garbetta A.; D'Antuono I.; Cardinali A.; Debellis L; Visconti A.; Martino N.A.

Titolo volume/Rivista

Archives of environmental contamination and toxicology

Anno di pubblicazione

2014

ISSN

0090-4341

ISBN

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