Abstract

Fumonisin B1 (FB1) is a mycotoxin produced by Fusarium species that exerts its toxic effect through interferencewith the sphingolipid pathway by inhibiting ceramide synthase. A FB1-dependent sperm toxicitywas reported in boars. No information on FB1-related reproductive toxicity in stallions, the most sensitiveanimal species, has been reported. The aim of the present study was to assess the in vitro toxicity ofFB1 on fresh and frozen-thawed equine spermatozoa by analyzing sperm viability, chromatin stability(SCSA) and reactive oxygen species (ROS) production by flow cytometry and sperm motility by CASA system.Fumonisin B1 did not affect viability of fresh spermatozoa after 2 h exposure up to 25 lM. Damageon sperm chromatin structure was observed only in one frozen sample after exposure up to2.5  105 lM FB1 without associated increase of ROS. Increase of ROS, at FB1 levels up to2.5  104 lM, was found on another frozen-thawed sperm sample, may be as a consequence of seminalplasma removal. At 7.5 and 15 lM, FB1 induced reduction of total and progressive motility.

Autore Pugliese

• Minervini Fiorenza

Tutti gli autori

• Minervini F.; Lacalandra G.M.; Filannino A.; Garbetta A.; Nicassio M.; Dell'Aquila M.E.; Visconti A.

Titolo volume/Rivista

In vitro toxicology

Anno di pubblicazione

2010

ISSN

0888-319X

ISBN

Non Disponibile

Numero di citazioni Wos

Nessuna citazione

Ultimo Aggiornamento Citazioni

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Numero di citazioni Scopus

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Ultimo Aggiornamento Citazioni

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Settori ERC

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Codici ASJC

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