Abstract

Tocopherols, collectively known as vitamin E, are lipophilic antioxidants, essential dietary components for mammals and exclusively synthesised by photosynthetic organisms. Of the four forms (alfa, beta, delta and gamma), alfa-tocopherol is the major vitamin E form present in green plant tissues, and has the highest vitamin E activity. Synthetic alfa-tocopherol, being a racemic mixture of eight different stereoisomers, always results less effective than the natural form (R,R,R)- a-tocopherol. This raises interest in obtaining this molecule from natural sources, such as plant cell cultures. Plant cell and tissue cultures are able to produce and accumulate valuable metabolites which can be used as food additives, nutraceuticals and pharmaceuticals. Sunflower cell cultures, growing under heterotrophic conditions, were exploited to establish a suitable in vitro production system of natural a-tocopherol. Optimization of culture conditions, precursor feeding and elicitor application were used to improve the tocopherol yields of these cultures. Furthermore, these cell cultures were useful to investigate the relationship between a-tocopherol biosynthesis and photomixotrophic culture conditions, revealing the possibility to enhance tocopherol production by favouring sunflower cell photosynthetic properties. The modulation of a-tocopherol levels in plant cell cultures can provide useful hints for a regulatory impact on tocopherol metabolism.

Autore Pugliese

• Caretto Sofia Pia Anna

Tutti gli autori

• Caretto S.; Nisi R.; Paradiso A.; De Gara L.

Titolo volume/Rivista

Molecular nutrition & food research

Anno di pubblicazione

2010

ISSN

1613-4125

ISBN

Non Disponibile

Numero di citazioni Wos

Nessuna citazione

Ultimo Aggiornamento Citazioni

Non Disponibile

Numero di citazioni Scopus

Non Disponibile

Ultimo Aggiornamento Citazioni

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Settori ERC

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Codici ASJC

Non Disponibile