Abstract

The involvement of thioredoxin/thioredoxinreductase system has been investigated in cerebellar granulecells (CGCs), a cellular system in which neurons areinduced in apoptosis by the physiological stimulus oflowering extracellular potassium. Clarifying the sequenceof events that occur during apoptosis is a critical issue as itcan lead to the identification of those key events that, ifblocked, can slow down or reverse the death process. Theresults reported in this work show that TrxR is involved inthe early phase of CGC apoptosis with an increase inactivity that coincides with the increased expression of theTrxR1 isoform and guarantees the maintenance of adequatelevel of Trx in its reduced, active form. However, in lateapoptosis, when about 50 % of cells are dead, partialproteolysis of TrxR1 by calpain occurs and the reduction ofTrxR1 mRNA, together with the overall decrease in TrxRactivity, contribute to increase the levels of the oxidizedform of Trx. When the reduced form of Trx is externallyadded to apoptotic cultures, a significant reduction in celldeath is achieved confirming that a well-functioning thioredoxin/thioredoxin reductase system is required for survivalof CGCs.

Autore Pugliese

• Atlante Anna , Bobba Antonella

Tutti gli autori

• Bobba A.; Casalino E.; Petragallo V.A.; Atlante A.

Titolo volume/Rivista

Apoptosis

Anno di pubblicazione

2014

ISSN

1360-8185

ISBN

Non Disponibile

Numero di citazioni Wos

Nessuna citazione

Ultimo Aggiornamento Citazioni

Non Disponibile

Numero di citazioni Scopus

Non Disponibile

Ultimo Aggiornamento Citazioni

Non Disponibile

Settori ERC

Non Disponibile

Codici ASJC

Non Disponibile