The potential of spectral and hyperspectral-imaging techniques for bacterial detection in food: A case study on lactic acid bacteria

Torna indietro

Abstract

Official methods for the detection of bacteria are based on culture techniques. These methods havelimitations such as time consumption, cost, detection limits and the impossibility to analyse a largenumber of samples. For these reasons, the development of rapid, low-cost and non-destructive analyticalmethods is a task of growing interest.In the present study, the capability of spectral and hyperspectral techniques to detect bacterial surfacecontamination was investigated preliminarily on gel cultures, and subsequently on sliced cookedham. In more detail, two species of lactic acid bacteria (LAB) were considered, namely Lactobacilluscurvatus and Lactobacillus sakei, both of which are responsible for common alterations in sliced cookedham.Three techniques were investigated, with different equipment, respectively: a macroscopic hyperspectralscanner operating in the NIR (10,470-5880 cm1) region, a FT-NIR spectrophotometer equippedwith a transmission arm as the sampling tool, working in the 12,500-5800 cm1 region, and a FT-MIRmicroscopy operating in the 4000-675 cm1 region.Multivariate exploratory data analysis, in particular principal component analysis (PCA), was appliedin order to extract useful information from original data and from hyperspectrograms. The results obtaineddemonstrate that the spectroscopic and imaging techniques investigated can represent an effectiveand sensitive tool to detect surface bacterial contamination in samples and, in particular, to recognisespecies to which bacteria belong.

Autore Pugliese

Tutti gli autori

  • G. Foca; C. Ferrari; A. Ulrici; G. Sciutto; S. Prati; S. Morandi; M. Brasca; P. Lavermicocca; S. Lanteri; P. Oliveri

Titolo volume/Rivista

Talanta

Anno di pubblicazione

2016

ISSN

0039-9140

ISBN

Non Disponibile

Numero di citazioni Wos

Nessuna citazione

Ultimo Aggiornamento Citazioni

Non Disponibile

Numero di citazioni Scopus

Non Disponibile

Ultimo Aggiornamento Citazioni

Non Disponibile

Settori ERC

Non Disponibile

Codici ASJC

Non Disponibile