Abstract

Programmed cell death can occur through two separate pathways caused by treatment of Saccharomyces cerevisiaewith acetic acid (AA-PCD), which differ from one another essentially with respect to their sensitivity to Nacetylcysteine(NAC) and to the role played by cytochrome c and metacaspase YCA1. Moreover, yeast can also undergomacroautophagy which occurs in NAC-insensitive manner. In order to gain some insight into the relationship betweenAA-PCD and macroautophagy use was made of WT and knock-out cells lacking YCA1 and/or cytochrome c. We showthat i. macroautophagy is modulated by YCA1 and by cytochrome c in a negative and positive manner, respectively, ii.the NAC-insensitive AA-PCD and macroautophagy differ from one another and iii. NAC-insensitive AA-PCD pathwaytakes place essentially without macroautophagy, even if the shift of extracellular pH to acidic values required for AA-PCDto occur leads itself to increased or decreased macroautophagy in YCA1 or cytochrome c-lacking cells.

Autore Pugliese

• Giannattasio Sergio

Tutti gli autori

• Antonacci L.; Guaragnella N.; Zdralevic M.; Passarella S.; Marra E. ; Giannattasio S.

Titolo volume/Rivista

Current pharmaceutical biotechnology

Anno di pubblicazione

2012

ISSN

1389-2010

ISBN

Non Disponibile

Numero di citazioni Wos

Nessuna citazione

Ultimo Aggiornamento Citazioni

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Numero di citazioni Scopus

Non Disponibile

Ultimo Aggiornamento Citazioni

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Settori ERC

Non Disponibile

Codici ASJC

Non Disponibile