Abstract

Liposomal formulations composed of phospholipids with different unsaturation degrees, head groups and at different cholesterol content have been tested for the encapsulation of Peptide Nucleic Acid (PNA) oligomers. The best loading capability (177 ?g, ER% = 87.2) was obtained for pure liposomes of phosphatidylglycerol (DOPG) with negatively charged head group. The insertion of a 10-20% of cholesterol in DOPG based liposomes provides a slight decrease (~160 ?g) of the PNA loading. On the other hand, the cholesterol addition (20-30%) slows down the PNA's release (~27%) in fetal bovine serum from the liposomal formulation. Based on the encapsulation and the release properties, PEGylated DOPG liposomes with a percentage of cholesterol of 10-20% are the optimal formulation for the loading of PNA-a210.

Autore Pugliese

• Saviano Michele

Tutti gli autori

• Ringhieri P.; Avitabile C.; Saviano M.; Morelli G.; Romanelli A.; Accardo A.

Titolo volume/Rivista

Colloids and surfaces. B, Biointerfaces

Anno di pubblicazione

2016

ISSN

0927-7765

ISBN

Non Disponibile

Numero di citazioni Wos

Nessuna citazione

Ultimo Aggiornamento Citazioni

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Numero di citazioni Scopus

Non Disponibile

Ultimo Aggiornamento Citazioni

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Settori ERC

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Codici ASJC

Non Disponibile