Abstract

A novel negative-stranded (ns) RNA virus associated with a severe citrus disease reported more than 80 years ago has been identified. Transmission electron microscopy showed that this novel virus, tentatively named citrus concave gum-associated virus, is flexuous and non-enveloped. Notwithstanding, its two genomic RNAs share structural features with members of the genus Phlebovirus, which are enveloped arthropod-transmitted viruses infecting mammals, and with a group of still unclassified phlebo-like viruses mainly infecting arthropods. CCGaV genomic RNAs code for an RNA-dependent RNA polymerase, a nucleocapsid protein and a putative movement protein showing structural and phylogenetic relationships with phlebo-like viruses, phleboviruses and the unrelated ophioviruses, respectively, thus providingintriguing evidence of a modular genome evolution. Phylogenetic reconstructions identified an invertebrate-restricted virus as themost likely ancestor of this virus, revealing that its adaptation to plants was independent from and possibly predated that of theother nsRNA plant viruses. These data are consistent with an evolutionary scenario in which trans-kingdom adaptationoccurred several times during the history of nsRNA viruses and followed different evolutionary pathways, in which genomic RNAsegments were gained or lost. The need to create a new genus for this bipartite nsRNA virus and the impact of the rapid andspecific detection methods developed here on citrus sanitation and certification are also discussed.

Autore Pugliese

• De Stradis Angelo , Navarro Ramirez Beatriz , Di Serio Francesco

Tutti gli autori

• Navarro B.; Minutolo M.; De Stradis A.; Palmisano F.; Alioto D.; Di Serio F.

Titolo volume/Rivista

Molecular plant pathology

Anno di pubblicazione

2018

ISSN

1464-6722

ISBN

Non Disponibile

Numero di citazioni Wos

Nessuna citazione

Ultimo Aggiornamento Citazioni

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Numero di citazioni Scopus

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Ultimo Aggiornamento Citazioni

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Settori ERC

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Codici ASJC

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