Abstract

The high-molecular-weight glutenin subunits (HMW-GS) represent a major component of the endospermstorage protein in the grains of wheat and its related species. Their technological importance results from their ready formationof intermolecular disulfide bonds, which underlie much of the visco-elasticity displayed by gluten and hence the processingquality of the flour. Here, sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis revealed that theChinese wheat cultivar Xiaoyanmai 7 formed four distinct HMW-GS,two of which are likely the product of a known allele atthe Glu-B1 locus, whereas the other two did not match any known HMW-GS.Acombined analysis based on reversed-phasehigh-performance liquid chromatography (RP-HPLC), N-terminal sequencing and mass spectrometry confirmed that the twonovel proteins were genuine HMW-GS. Inspection of the DNA sequences showed that one of the novel HMW-GS wasencoded by an x-type and the other by a y-type secalin gene. A karyotypic analysis confirmed that six of the seven pairs ofXiaoyanmai 7'sD genome chromosomes (the exception was chromosome 2D) had been replaced by rye chromosomes. They-type HMW secalin present in Xiaoyanmai 7 differed from the standard By and Dy HWM-GS by the presence of anadditional cysteine residue in its C-terminal domain.

Autore Pugliese

• Margiotta Benedetta

Tutti gli autori

• Feng B.; Sestili F.; Masci S.; Margiotta B.; Xu Z.; Yang Z.; Xiang C.; Zhou C.; Lafiandra D.; Wang T.

Titolo volume/Rivista

Crop & pasture science

Anno di pubblicazione

2016

ISSN

1836-0947

ISBN

Non Disponibile

Numero di citazioni Wos

Nessuna citazione

Ultimo Aggiornamento Citazioni

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Numero di citazioni Scopus

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Ultimo Aggiornamento Citazioni

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Settori ERC

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Codici ASJC

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