Abstract

Crystallization still represents the bottleneck in the process of protein structure determination at high resolution, despite high-throughput structural genomics programs require optimized crystallization strategies regarding crystal quality, time, success rate, reproducibility and used protein amount. On the other side, the development of suitable materials for controlled heterogeneous nucleation might facilitate biomacromolecular crystallization in a variety of experimental conditions which are not conventionally fruitful. Here we show the possibility to fabricate hydrogel membranes displaying controlled chemical composition and nanostructure and to use them as heterogeneous supports for biomacromolecular crystallization. Diverse gel morphologies were obtained by controlling phase separation kinetics during gel layer formation on membrane support. These composite materials were found to increase the efficiency of the crystallization process so that crystals with enhanced diffraction properties were produced at lower protein concentration than conventional technique, thus affording the possibility to improve current approaches to protein crystallization and to be adapted to specific targets.

Autore Pugliese

• Caliandro Rocco

Tutti gli autori

• Di Profio G.; Polino M.; Nicoletta F. P.; Belviso B. D.; Caliandro R.; Fontananova E.; De Filpo G.; Curcio E.; Drioli E.

Titolo volume/Rivista

Advanced functional materials

Anno di pubblicazione

2014

ISSN

1616-301X

ISBN

Non Disponibile

Numero di citazioni Wos

Nessuna citazione

Ultimo Aggiornamento Citazioni

Non Disponibile

Numero di citazioni Scopus

Non Disponibile

Ultimo Aggiornamento Citazioni

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Settori ERC

Non Disponibile

Codici ASJC

Non Disponibile