Synthesis of a l-lysine-based alternate alpha,epsilon-peptide: a novel linear polycation with nucleic acids-binding ability

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Abstract

A novel nucleic acid compaction device based on a positivelychargedalpha,epsilonpolyllysinewasrealized for the first time. The polycationic peptide was obtained by assembling Fmoc and Boc orthogonallyprotected llysinemonomers by solid phase synthesis. The route to the novel polycation is veryfast and convenient because it allows for the obtainment of the desired product in few synthetic stepsexclusively employing Fmoc chemistry. The purification of the polyaminoacid was performed easilyby RPHPLCon C18 stationary phase while ESIMSanalysis confirmed the identity of the novel basicmolecule. The structural characteristics of the novel watersolublepeptide, as well as the interaction withdeoxyribonucleic acid (DNA) and ribonucleic acid (RNA) have been investigated by circular dichroism (CD)and ultraviolet (UV) spectroscopy.

Autore Pugliese

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  • Moccia M.; Roviello G.N.; Bucci E.M.; Pedone C.; Saviano M.

Titolo volume/Rivista

International journal of pharmaceutics

Anno di pubblicazione

2010

ISSN

0378-5173

ISBN

Non Disponibile

Numero di citazioni Wos

Nessuna citazione

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Numero di citazioni Scopus

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Ultimo Aggiornamento Citazioni

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Settori ERC

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Codici ASJC

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