Abstract

Five viroid species have been reported fromgrapevine. Hop stunt viroid (HSVd) and Grapevine yellowspeckle viroid 1 (GYSVd-1) are distributed worldwide,whereas Grapevine yellow speckle viroid 2(GYSVd-2), Australian grapevine viroid (AGVd) andCitrus exocortis viroid (CEVd) are found only sporadically.However, the presence of AGVd and GYSVd-2 inseveral countries, including China, Turkey and Tunisia,suggests a wider dissemination, possibly also in Europe,where AGVd has never been found and GYSVd-2 hasbeen occasionally identified in Italy. Taking advantage ofa multiplex RT-PCR assay recently developed for detectingsimultaneously these five viroids, vines growing inItaly in commercial vineyards and germplasm collectionswere surveyed. Besides confirming the widespreadpresence of HSVd and GYSVd-1 in the field, GYSVd-2and/or AGVd were identified in two grapevine tablecultivars (Sultanina Bianca and Red Globe) from germplasmcollections. Tests extended to vines cultivated insouthern Italy confirmed the presence of both viroids,which were further characterized. No major sequencedivergences between the AGVd and GYSVd-2 variantsfrom Italy and those previously described from othercountries were observed. Phylogenetic analysis supportedthe close relationships among AGVd variants from Italy,Tunisia and Australia. To our knowledge this is the firstreport of AGVd in Europe and the first molecular characterizationof GYSVd-2 isolates from a European country.

Autore Pugliese

• Navarro Ramirez Beatriz , Di Serio Francesco , La Notte Pierfederico

Tutti gli autori

• Gambino G.; Navarro B.; Torchetti E.M.; La Notte P.; Schneider A.; Mannini F.; Di Serio F.

Titolo volume/Rivista

European journal of plant pathology

Anno di pubblicazione

2014

ISSN

0929-1873

ISBN

Non Disponibile

Numero di citazioni Wos

Nessuna citazione

Ultimo Aggiornamento Citazioni

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Numero di citazioni Scopus

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Ultimo Aggiornamento Citazioni

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Settori ERC

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Codici ASJC

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