Abstract

Celiac disease is an immune-mediated enteropathy precipitated by the ingestion of gluten-containing foodsin genetically predisposed children and adults. After a positive diagnosis for celiac disease, the only availabletreatment is to adopt a gluten-free diet, and evaluation of the absence of gluten in foods is crucial for the health ofceliac patients. In the present study, a recombinant glutamine-binding protein (GlnBP) from Escherichia coli showedits ability to recognize peptides deriving from digested wheat flour. GlnBP and the commercially available 4F3monoclonal antibody, raised against a region of the ?-gliadin peptide 33-mer from wheat, demonstrated the abilityto detect gliadin extracted from wheat flour. Recombinant GlnBP and 4F3 monoclonal antibody were used as newcapture agents for the development of a protein chip able to detect gluten in foods. The protein microarray systemhas proven to detect the presence of gliadin in a range of concentrations between 500 and 5 ppm.

Autore Pugliese

• Bleve Gianluca , Mita Giovanni

Tutti gli autori

• F. Cimaglia; G. Potente; M. Chiesa; G. Mita; G. Bleve

Titolo volume/Rivista

Journal of proteomics and bioinformatics

Anno di pubblicazione

2014

ISSN

0974-276X

ISBN

Non Disponibile

Numero di citazioni Wos

Nessuna citazione

Ultimo Aggiornamento Citazioni

Non Disponibile

Numero di citazioni Scopus

Non Disponibile

Ultimo Aggiornamento Citazioni

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Settori ERC

Non Disponibile

Codici ASJC

Non Disponibile