Abstract

The aim of this study was to evaluate, by computer-assisted analysis, the motility parameters of cryopreserved sea bream spermatozoa after prolonged storage (up to 5 years) in liquidnitrogen in comparison to the performances of fresh semen and of semen thawed 1 month after freezing (cryopreservation medium: 1% NaCl containing 5% DMSO; freezing rate: 10°C min
1; stored in liquid nitrogen). Semen samples were thawed 1 month and 5 years after cryopreservation. Sperm motility was analyzed by means of the Sperm Class Analyzer
(SCA, Microptic, Barcelona, Spain). The percentages of motile sperm and rapid sperm curvilinear velocity >100 lm s
1 only), and the curvilinear, straight-line and average path velocities (lm s
1) were evaluated. The percentages of total motile and rapid sperm, as well the relative velocity levels, were slightlylower in thawed semen than in fresh semen (for example, 85 vs 95% for total motile sperm; 70 vs 80% for rapid sperm; 200 vs 300 lm s
1 for VCLt; 250 vs 350 lm s
1 for VCLr). Data of all trials did not differ in relation to storage time. It can therefore be concluded that long-term storage of large amountsof cryopreserved semen was homogeneous, providing high quality sea bream semen for use in fertilization trials in both aquaculture and laboratory research.

Autore Pugliese

• Pelosi Sergio , Fabbrocini Adele , D'adamo Raffaele

Tutti gli autori

• A. Fabbrocini; R. D'Adamo; S. Pelosi; L. F. J. Oliveira; F. Del Prete; F. Silvestri; V. Vitiello; G. Sansone

Titolo volume/Rivista

Journal of applied ichthyology

Anno di pubblicazione

2014

ISSN

0175-8659

ISBN

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Numero di citazioni Wos

Nessuna citazione

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Numero di citazioni Scopus

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Settori ERC

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Codici ASJC

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