Abstract

Cryopreservation is a valuable tool for aquacultureas it provides continuous seed production, regardlessof the spawning season of the brood stock. Theselection of a suitable cryoprotectant with low toxicityand high water solubility is important to avoidmembrane injuries and intracellular ice crystallization.This study has been aimed at the assessment ofthe toxic effects of two usually applied cryoprotectants,1-2 propylene glycol (PG) and methanol(MetOH), on spermatozoa of the of lion-paw scallopNodipecten nodosus, by evaluating the sperm motilityand the development of D larvae after fertilizationprocedure. Sperm was exposed at room temperature(22°C) for 10, 20 and 30 min to different concentrationranges of two cryoprotectants. Regardingthe sperm motility, PG5%, PG7%, MetOH4% andMetOH6% did not show differences compared tocontrol (semen incubated in seawater) (P < 0.05).The development of D larvae was not affected by theexposition to PG5%, MetOH 4% and MetOH 6%.These results indicate the potential use of both cryoprotectantsfor cryopreservation procedures.

Autore Pugliese

• Fabbrocini Adele

Tutti gli autori

• Silvestri F.; Vitiello V.; Del Prete F.; Fabbrocini A.; Ferreira J.F.; Sansone G.

Titolo volume/Rivista

Aquaculture research

Anno di pubblicazione

2014

ISSN

1355-557X

ISBN

Non Disponibile

Numero di citazioni Wos

Nessuna citazione

Ultimo Aggiornamento Citazioni

Non Disponibile

Numero di citazioni Scopus

Non Disponibile

Ultimo Aggiornamento Citazioni

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Settori ERC

Non Disponibile

Codici ASJC

Non Disponibile