Abstract

Fruits and vegetables contain a number of components (polyphenols, carotenoids, vitamins, folate, minerals such as magnesium, calcium, silicon and other compounds) that could affect the bone health. Considering the biological activities induced by silicon (antioxidant activity, increased mineralization of bone, development of connective tissue), this study aims to compare the biological activity induced by the silicon biofortificated and unbiofortificated vegetables (tatsoi, mizuna, purslane, Swiss chard and chicory) respect to the commercial silicon supplement. In addition, the assessment of silicon bioaccessibility, bioavailability and biological activity in human intestinal cell line (Caco-2) and human osteoblasts (hOBs) were performed. After in vitro gastrointestinal digestion of plant edible leaves and the silicon supplementation, the silicon bioaccessibility ranged from 7.01 to 13.22 mg/L. On Caco-2 cell line, the antioxidant activity of the bioaccessible fraction was assessed as reduction of induced ROS levels and ranged from 25 to 44% respect to stimulated control. The silicon biofortification significantly (p<0.001) improved the antioxidant activity induced by the vegetables. The bioavailability of digested samples, evaluated on Caco-2 differentiated cell line, ranged from 237 to 440 µg/L with percentages ranged from 27% to 61% in relation to the considered vegetable species. The expression of collagen type 1 and alkaline phosphatase induced by the bioavailable fraction after 12 and 36 h of treatment was evaluated in hOBs by real-time PCR. The bioavailable fraction of tatsoi, purslane and Swiss chard (biofortificated leafy vegetables), improved (after 36 hours of incubation) the expression of collagen type 1 and alkaline phosphatase with respect to the silicon supplement and unbiofortifated vegetables in hOBs.

Autore Pugliese

• Cardinali Angela , Minervini Fiorenza , Serio Francesco

Tutti gli autori

• D'Imperio M.; Brunetti G. ; Gigante I. ; Serio F. ; Santamaria P. ; Cardinali A. ; Colucci S. ; Minervini F.

Titolo volume/Rivista

Non Disponibile

Anno di pubblicazione

2015

ISSN

Non Disponibile

ISBN

Non Disponibile

Numero di citazioni Wos

Nessuna citazione

Ultimo Aggiornamento Citazioni

Non Disponibile

Numero di citazioni Scopus

Non Disponibile

Ultimo Aggiornamento Citazioni

Non Disponibile

Settori ERC

Non Disponibile

Codici ASJC

Non Disponibile