Sequencing an unconventional virus genome: the mulberry badnavirus-1 case.

Torna indietro

Abstract

Mulberry is a deciduous tree belonging to the Moraceae family. Although its economical importance and spreading all over the world, due mainly to the domesticated silkworm (Bombyx mori L.) breeding, to date, only few information are available about viral and virus-like diseases affecting this plant. In 2012 a small fragment of a Badnavirus DNA genome was sequenced after a DOP-PCR assay conducted on a mulberry plant, originated from Lebanon, showing symptoms of leaf mottling and vein yellowing. The virus, whose particles were observed with electron microscopy from a partially purified preparation and in tissue thin sections, was provisionally named Mulberry badnavirus-1 (MBV-1). Since different attempts of completing the full-length genome sequence through a conventional approach failed, a small RNA library was constructed for deep sequencing and run according to Illumina protocol ssRNAs analysis allowed the design of a set of primers used in PCR for the achievement of the full length sequence. The complete genome contains all the sequence features and the characteristic functional domains of the genus Badnavirus (highest nucleotide similarity shared with FBV-1 at 54%). By contrast to the badnaviruses, with genomes encoding for 3-4 ORFs, MBV-1 resembles genome organization of Petunia vein clearing virus (PVCV), which bears a single ORF. The study of the distribution of sRNA on the MBV-1 complete genome showed a tidy prevalence of 21- and 22-nt reads, differently from other viruses in the Caulimoviridae family, featuring a nuclear replication and typically supporting an accumulation of the 24-nt sRNAs involved in methylation processes.

Autore Pugliese

Tutti gli autori

  • Chiumenti M.; Morelli M.; Elbeaino T.; Stavolone L.; De Stradis A.; Minafra A.; Martelli G.P.

Titolo volume/Rivista

Journal of plant pathology

Anno di pubblicazione

2014

ISSN

1125-4653

ISBN

Non Disponibile

Numero di citazioni Wos

Nessuna citazione

Ultimo Aggiornamento Citazioni

Non Disponibile

Numero di citazioni Scopus

Non Disponibile

Ultimo Aggiornamento Citazioni

Non Disponibile

Settori ERC

Non Disponibile

Codici ASJC

Non Disponibile