Abstract

We crystallised SdsA, a sodium dodecyl sulphate hydrolase, from Pseudomonas aeruginosa in three different crystal polymorphs and determined their three-dimensional structure. The different polymorphs present different crystal packing habits. One of the polymorphs suggests the existence of a tetramer, an oligomeric state not observed previously, while the crystal packing of the remaining two polymorphs obstructs the active site entrance but stabilizes flexible regions of the protein. Non-conventional crystallisation methods that minimise convection such as counter-diffusion in polyvinyl alcohol gel coupled with the influence of a 500 MHz (10.2 Tesla) magnetic field were necessary to isolate the poorest diffracting polymorph and increase its internal order to determine its structure by X-ray diffraction. Our results show the effectiveness of non-conventional crystallographic methods to isolate different crystal polymorphs.

Autore Pugliese

• Siliqi Dritan

Tutti gli autori

• E. De la Mora; E. Flores-Hernandez; J. Jankocic; V. Stojanoff; D. Siliqi; N. Sanchez-Puig ; A. Moreno

Titolo volume/Rivista

Journal of applied crystallography

Anno di pubblicazione

2015

ISSN

0021-8898

ISBN

Non Disponibile

Numero di citazioni Wos

Nessuna citazione

Ultimo Aggiornamento Citazioni

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Numero di citazioni Scopus

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Ultimo Aggiornamento Citazioni

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Settori ERC

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Codici ASJC

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