Abstract

Osteoarthritis (OA), among other bone pathologies, is expected to determine supramolecular changes at the level of the mineralized collagen fiber. In a proof-of-principle study, bone biopsies were collected from six coxarthritis-affected patients, aged 62-87 years, during hip prosthesis implant surgery, sliced down to 100 mm-thick tissues, and investigated using scanning small-angle and wide-angle X-ray scattering (SAXS and WAXS) transmission microscopy. A multimodal imaging evaluation of the SAXS and WAXS data, combined with principal component and canonical correlation analyses, allowed the transformation of the raw data into microscopy images and inspection of the nanoscale structure of the mineralized collagen fibers across mm 2 tissue areas. The combined scanning SAXS and WAXS microscopy is shown to be a suitable choice for characterizing and quantifying the nanostructural properties of collagen over extended areas. The results suggest the existence of a correlation between age and cross-linking-induced rigidity of collagen fibers.

Autore Pugliese

• Giannini Cinzia , Altamura Davide , Siliqi Dritan , De Caro Liberato , Ladisa Massimo , Sibillano Teresa

Tutti gli autori

• Giannini C.; Siliqi D.; Ladisa M.; Altamura D.; Diaz A.; Beraudi A.; Sibillano T.; De Caro L.; Stea S.; Baruffaldi F.; Bunk O.

Titolo volume/Rivista

Journal of applied crystallography

Anno di pubblicazione

2014

ISSN

0021-8898

ISBN

Non Disponibile

Numero di citazioni Wos

Nessuna citazione

Ultimo Aggiornamento Citazioni

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Numero di citazioni Scopus

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Ultimo Aggiornamento Citazioni

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Settori ERC

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Codici ASJC

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