Abstract

PHA synthases (PhaC) are grouped into four classes based on the kinetics and mechanisms of reaction. The grouping of PhaC enzymes into four classes is dependent on substrate specificity, according to the preference in forming short chain length (scl) or medium chain length (mcl) polymers: class I, class III, and class IV produce scl-PHAs depending on propionate, butyrate, valerate and hexanoate precursors, while class II phaC synthesize mcl-PHAs based on the alkane (C6 to C14) precursors. PHA synthases of class I, in particular PhaCCs from Chromobacterium USM2 and PhaCCn/RePhaC1 from Cupriavidus necator/Ralstonia eutropha, have been analysed and the crystal structures of the C-domains have been determined. PhaCCn/RePhaC1 was also studied by X-ray absorption fine-structure (XAFS) analysis. Models have been proposed for dimerization,catalysis mechanism, substrate recognition and affinity, product formation, and product egressroute. The assays based on amino acid substitution by mutagenesis have been useful to validatethe hypothesis on the role of amino acids in catalysis and in accommodation of bulky substrates,and for the synthesis of PHB copolymers and medium-chain-length PHA polymers with optimizedchemical properties.

Autore Pugliese

• Poltronieri Palmiro

Tutti gli autori

• Mezzolla V.; D'Urso O.F.; Poltronieri P.

Titolo volume/Rivista

Polymers

Anno di pubblicazione

2018

ISSN

2073-4360

ISBN

Non Disponibile

Numero di citazioni Wos

Nessuna citazione

Ultimo Aggiornamento Citazioni

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Numero di citazioni Scopus

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Ultimo Aggiornamento Citazioni

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Settori ERC

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Codici ASJC

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