Abstract

Carcinoma progression is associated with the loss of epithelial features, and the acquisitionof a mesenchymal phenotype by tumour cells. Herein we show that exposure of MCF-7cells to epidermal growth factor (EGF) resulted in morphological alterations characteristicof epithelial-to-mesenchymal transition (EMT). EGF treatment resulted in increased motilityalong with an up-regulation of transcription factors Slug, Zeb1, Zeb2, and mesenchymalmarkers Vimentin and N-cadherin.Treatment of MCF-7 cells with a combined stimulation of EGF and resveratrol, a naturallyoccurring stilbene with antitumor properties, failed to alter cell morphology, motility andoverexpression of EMT markers induced by EGF. Using specific chemical inhibitors, wedemonstrated that EGF-induced EMT is mediated by extracellular signal-regulated kinase1/2 (ERK 1/2) signalling pathway and that resveratrol is able to repress EGF-induced ERKactivation.In summary, these data provide new evidence of the inhibitory effect of resveratrol onEGF-induced EMT cell transformation.

Autore Pugliese

• Santino Angelo , Giovinazzo Giovanna

Tutti gli autori

• Vergara D.; Valente C.M.; Tinelli A.; Siciliano C.; Lorusso V.; Acierno R.; Giovinazzo G.; Santino A.; Storelli C.; Maffia M.

Titolo volume/Rivista

Cancer letters

Anno di pubblicazione

2011

ISSN

0304-3835

ISBN

Non Disponibile

Numero di citazioni Wos

Nessuna citazione

Ultimo Aggiornamento Citazioni

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Numero di citazioni Scopus

Non Disponibile

Ultimo Aggiornamento Citazioni

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Settori ERC

Non Disponibile

Codici ASJC

Non Disponibile