Abstract

Reliable methods are needed for detection ofallergenic milk proteins in complex food matrixes.The feasibility of an LC/high-resolution MS methodfor the analysis of milk proteins in a thermallyprocessed model food (incurred cookies) and inwhite wine spiked, respectively, with milk powderand caseinate is described. Detection of milkproteins was based on the identification of uniquepeptides in the tryptic digests of cookie/wineextracts using an RP-HPLC separation coupled toan Exactive(TM) nonhybrid mass spectrometer usingOrbitrap technology. The extremely high massaccuracy and resolution provided by the Orbitrapanalyzer allowed a fast preliminary identification offour previously proposed peptide markers ofcaseins using only accurate values of the m/z oftheir ions. No interference was observed, despitethe complexity of the analyzed matrixes. Moreover,the availability of a high- energy, collisionallyactivated dissociation cell integrated in the massspectrometer enabled acquisition of peptideMS/MS-like spectra through post-sourcefragmentation. Confirmation of peptide markeridentity could then be achieved by a comparisonbetween experimental and predicted product ions.The described method shows the great potential ofOrbitrap MS as a reliable technique in the field ofprotein allergen detection once the peptidemarkers are identified.

Autore Pugliese

• Monaci Linda

Tutti gli autori

• Monaci L.; Losito I.; Palmisano F.; Visconti A.

Titolo volume/Rivista

Journal of AOAC International

Anno di pubblicazione

2011

ISSN

1060-3271

ISBN

Non Disponibile

Numero di citazioni Wos

Nessuna citazione

Ultimo Aggiornamento Citazioni

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Numero di citazioni Scopus

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Ultimo Aggiornamento Citazioni

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Settori ERC

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Codici ASJC

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