Abstract

A study was carried out for identification and detection of Phytophthora spp. from soil and plant samples, collected fromSolanaceous crops in Egypt and Italy. The samples were screened with specific and universal primers of Phytophthora 18S,ITS1-ITS2 and 28S regions, followed by PCR product sequencing. The Phytophthora spp. detected were P. infestans(Egypt) and P. parasitica (Italy). A molecular beacon probe was also developed based on the avr3a gene of P. infestans todetect a variant associated with virulence traits. The probe was suitable for avr3a allele identification from P. infestans andalso from P. parasitica PCR products.

Autore Pugliese

• Finetti Sialer Mariella Matilde

Tutti gli autori

• Tolba S.R.T.; Finetti Sialer M.M.; Rosso L.C.; Moustafa M.M.A.; Ruggeri C.; El-Shawaf I.I.S.; Ciancio A.

Titolo volume/Rivista

Journal of plant diseases and protection

Anno di pubblicazione

2018

ISSN

1861-3837

ISBN

Non Disponibile

Numero di citazioni Wos

Nessuna citazione

Ultimo Aggiornamento Citazioni

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Numero di citazioni Scopus

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Ultimo Aggiornamento Citazioni

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Settori ERC

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Codici ASJC

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